A couple more good notes about Twitter is to make sure you are generating lots and lots of good content. Even hire a social media manager if you are having trouble with staying on top of adding new tweets or responding to fans/followers (this is very cheap to do nowadays). One cool way I have seen of generating lots of good content is using a combination of Google reader and Hootsuite. Add a bunch of industry related blogs to your Google reader then tweet scheduled links to them using Hootsuite.
Another practice you will want to take up is following people who known to follow back and actively participate in the Twitter community regardless of what your business specialty is.
A final note is you will want to study and try all out all the different Twitter tools on the market to see what works best (there are 100's to go through, some very valuable). Also twitter exchanges can be key as popularity and image is a large part of the Twitter battle.
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